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KMID : 0380020060210060451
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 6 p.451 ~ p.455
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Isolation and Characterization of An Alcohol Fermentation Strain from Anaerobic Acid Fermentor to Treat Food Wastes
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Kim Jung-Kon
Yoo Jin-Cheol
Kim Seong-Jun
Han Gui-Hwan
Seong Chi-Nam
Kim Si-Wouk
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Abstract
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An efficient pilot scale (10 ton) three-stage methane fermentation system to digest food waste has been developed in this laboratory. This system consisted of three stages: semianaerobic hydrolysis, anaerobic acidogenesis and strictly anaerobic methanogenesis. From the secondary acidogenesis reactor, a novel strain KA4 responsible for alcohol fermentation was isolated and characterized. The cell was oval and its dimension was . This strain was identified as Saccharomyces cerevisiae KA4 by 26S rDNA D1/D2 rDNA sequence. Optimal culture temperature was . Cells were tolerant to 5% (v/v) ethanol concentration, however, were inhibited significantly by higher ethanol concentration up to 7%. The strain could grow well up to 50% (w/v) initial glucose concentration in the YM liquid medium, however, optimal concentration for ethanol fermentation was 10%. It could produce ethanol in a broad initial pH range from 4 to 10, and optimal pH was 6. In this condition, the strain converted 10% glucose to 7.4% ethanol during 24 hr, and ethanol yield was estimated to be 2.87 moi EtOH/mol glucose.
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KEYWORD
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Alcohol fermentation, Saccharomyces cerevisiae KA4, three-stage methane fermentation system
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